Mechanisms of calcium absorption by anterior and posterior segments of the intestinal tract of juvenile lake sturgeon.
Identifieur interne : 000278 ( Main/Exploration ); précédent : 000277; suivant : 000279Mechanisms of calcium absorption by anterior and posterior segments of the intestinal tract of juvenile lake sturgeon.
Auteurs : Janet Genz [États-Unis] ; Benjamin Carriere ; W Gary AndersonSource :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [ 1531-4332 ] ; 2013.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Calcium.
- chemical , pharmacology : Calcium Channel Blockers, Ruthenium Red, Trifluoperazine.
- drug effects : Intestines.
- metabolism : Fishes, Intestines.
- Animals, In Vitro Techniques, Intestinal Absorption, Osmolar Concentration.
Abstract
Rapid growth in juvenile fish increases calcium demand, and the intestine may play a role in calcium homeostasis at this life stage, in addition to branchial and renal transport. This study examined calcium flux in the gastrointestinal tract (GIT) of freshwater juvenile lake sturgeon acclimated to 0.14, 0.34, and 2.26mmol L(-1) environmental calcium. Net Ca(2+) flux did not differ due to environmental [Ca(2+)] in either the anterior or posterior intestine. Blocking the apical epithelial calcium channel (ECaC) with ruthenium red (RR, 8.5μmol L(-1)) significantly decreased Ca(2+) influx in the anterior intestine, but exposure to the plasma membrane Ca(2+)-ATP-ase (PMCA) inhibitor trifluoperazine (TFP, 10mmol L(-1)) had no effect at any environmental [Ca(2+)], nor did inhibition of the Na(+)-Ca(2+) exchanger (NCX) with KB-R7943 (10μmol L(-1)). Neither RR nor TFP affected Ca(2+) uptake by the posterior intestine in any of the treatment groups, but KB-R7943 reduced net calcium flux in the posterior intestine at all environmental [Ca(2+)]. Thus, basolateral Ca(2+) influx in the posterior GIT of lake sturgeon relies more heavily on NCX than PMCA. Furthermore, the differing pharmacological effects in the anterior and posterior intestine suggest that the dominant transporters responsible for calcium uptake vary over the length of the GIT in lake sturgeon.
DOI: 10.1016/j.cbpa.2013.06.033
PubMed: 23831300
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000237
- to stream PubMed, to step Curation: 000237
- to stream PubMed, to step Checkpoint: 000237
- to stream Ncbi, to step Merge: 000487
- to stream Ncbi, to step Curation: 000487
- to stream Ncbi, to step Checkpoint: 000487
- to stream Main, to step Merge: 000279
- to stream Main, to step Curation: 000278
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mechanisms of calcium absorption by anterior and posterior segments of the intestinal tract of juvenile lake sturgeon.</title><author><name sortKey="Genz, Janet" sort="Genz, Janet" uniqKey="Genz J" first="Janet" last="Genz">Janet Genz</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, University of West Georgia, Carrollton, GA 30118, USA. jgenz@westga.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of West Georgia, Carrollton, GA 30118</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Carriere, Benjamin" sort="Carriere, Benjamin" uniqKey="Carriere B" first="Benjamin" last="Carriere">Benjamin Carriere</name></author><author><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23831300</idno><idno type="pmid">23831300</idno><idno type="doi">10.1016/j.cbpa.2013.06.033</idno><idno type="wicri:Area/PubMed/Corpus">000237</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000237</idno><idno type="wicri:Area/PubMed/Curation">000237</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000237</idno><idno type="wicri:Area/PubMed/Checkpoint">000237</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000237</idno><idno type="wicri:Area/Ncbi/Merge">000487</idno><idno type="wicri:Area/Ncbi/Curation">000487</idno><idno type="wicri:Area/Ncbi/Checkpoint">000487</idno><idno type="wicri:Area/Main/Merge">000279</idno><idno type="wicri:Area/Main/Curation">000278</idno><idno type="wicri:Area/Main/Exploration">000278</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mechanisms of calcium absorption by anterior and posterior segments of the intestinal tract of juvenile lake sturgeon.</title><author><name sortKey="Genz, Janet" sort="Genz, Janet" uniqKey="Genz J" first="Janet" last="Genz">Janet Genz</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, University of West Georgia, Carrollton, GA 30118, USA. jgenz@westga.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of West Georgia, Carrollton, GA 30118</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Carriere, Benjamin" sort="Carriere, Benjamin" uniqKey="Carriere B" first="Benjamin" last="Carriere">Benjamin Carriere</name></author><author><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</title><idno type="eISSN">1531-4332</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Calcium (metabolism)</term><term>Calcium Channel Blockers (pharmacology)</term><term>Fishes (metabolism)</term><term>In Vitro Techniques</term><term>Intestinal Absorption</term><term>Intestines (drug effects)</term><term>Intestines (metabolism)</term><term>Osmolar Concentration</term><term>Ruthenium Red (pharmacology)</term><term>Trifluoperazine (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium Channel Blockers</term><term>Ruthenium Red</term><term>Trifluoperazine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Intestines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Intestines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>In Vitro Techniques</term><term>Intestinal Absorption</term><term>Osmolar Concentration</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rapid growth in juvenile fish increases calcium demand, and the intestine may play a role in calcium homeostasis at this life stage, in addition to branchial and renal transport. This study examined calcium flux in the gastrointestinal tract (GIT) of freshwater juvenile lake sturgeon acclimated to 0.14, 0.34, and 2.26mmol L(-1) environmental calcium. Net Ca(2+) flux did not differ due to environmental [Ca(2+)] in either the anterior or posterior intestine. Blocking the apical epithelial calcium channel (ECaC) with ruthenium red (RR, 8.5μmol L(-1)) significantly decreased Ca(2+) influx in the anterior intestine, but exposure to the plasma membrane Ca(2+)-ATP-ase (PMCA) inhibitor trifluoperazine (TFP, 10mmol L(-1)) had no effect at any environmental [Ca(2+)], nor did inhibition of the Na(+)-Ca(2+) exchanger (NCX) with KB-R7943 (10μmol L(-1)). Neither RR nor TFP affected Ca(2+) uptake by the posterior intestine in any of the treatment groups, but KB-R7943 reduced net calcium flux in the posterior intestine at all environmental [Ca(2+)]. Thus, basolateral Ca(2+) influx in the posterior GIT of lake sturgeon relies more heavily on NCX than PMCA. Furthermore, the differing pharmacological effects in the anterior and posterior intestine suggest that the dominant transporters responsible for calcium uptake vary over the length of the GIT in lake sturgeon.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name><name sortKey="Carriere, Benjamin" sort="Carriere, Benjamin" uniqKey="Carriere B" first="Benjamin" last="Carriere">Benjamin Carriere</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Genz, Janet" sort="Genz, Janet" uniqKey="Genz J" first="Janet" last="Genz">Janet Genz</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000278 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000278 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23831300
|texte= Mechanisms of calcium absorption by anterior and posterior segments of the intestinal tract of juvenile lake sturgeon.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23831300" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |